Urinalysis Hands on Lab Assignment
Introduction:
The million nephrons in each of your kidneys form urine and which allows the body to get rid of metabolic wastes from blood and maintain homeostasis by regulating blood pH, water volume and ion concentrations in blood. Urinalysis is a standard test that can determine if the urine formation is normal or abnormal. In doing this lab we can determine the volume of urine, chemical and physical properties, and microscopic composition along with levels of some metabolic waste materials in blood.

Pre-activity
There are three processes for making urine, glomerular filtration, tubular reabsorption, and tubular secretion. Below, for each of the processes below, define them and describe where they occur in the nephron.

1. Glomerular Filtration

2. Tubular Reabsorption

3. Tubular Secretion

Activity 1: The Nephron
Make a hand drawn picture of the nephron, make sure to include all of the structures and information listed below.

A. Renal corpuscle

B. Renal tubule

C. Location of glomerular filtration

D. Location and directional arrow of tubular reabsorption

E. Location and directional arrow of tubular secretion

F. Proximal convoluted tubule

G. Distal convoluted tubule

H. Glomerular capsule (Bowman’s capsule)

I. Collecting duct

J. Ascending loop of Henle

K. Descending loop of Henle

Post your labeled picture here.
Activity 2: Renal Reabsorption and Secretion
Using pages 970-988 in your lecture book and these videos Urinary System 1 (opens window) and Urinary System 2 (opens window) use the table below to predict the difference in osmolarity between the filtrate in the glomerular capsule and the other regions of the nephron. Will it be higher, lower, the same as, or dependent on hormones? For example, looking at the first structure in the table, does the filtrate found at the beginning of the proximal convoluted tubule have a higher/lower/same as the filtrate found in the glomerular capsule or does it depend on the hormones present?

Nephron structure

Glomerular Capsule

Beginning of proximal convoluted tubule

End of proximal convoluted tubule

End of the descending limb of the Loop of Henle

End of the ascending limb of the Loop of Henle

Distal convoluted tubule

Collecting duct

Please READ understand and complete the full assignment!!